Biofilm-Modelle - MDC Buhmann

Modelling biofilms

In order to investigate antimicrobial or even anti-biofilm properties of materials, laboratory model systems are needed that reflect the application situation.

Biofilm model systems for the study of antimicrobial materials.

Buhmann, M. T., Stiefel, P., Maniura-Weber, K., & Ren, Q. (2016). In vitro biofilm models for device-related infections. Trends in biotechnology, 34(12), 945-948.

Adithi R. Varadarajan, Raymond N. Allan, Jules D. P. Valentin, Olga E. Castañeda Ocampo, Vincent Somerville, Franziska Pietsch, Matthias T. Buhmann, Jonathan West, Paul J. Skipp, Henny C. van der Mei, Qun Ren, Frank Schreiber, Jeremy S. Webb & Christian H. Ahrens (2020). An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in Pseudomonas aeruginosa MPAO1. NPJ biofilms and microbiomes, 6(1), 1-17.https://www.nature.com/articles/s41522-020-00154-8

Bacterial adhesion

The influence of material properties on bacterial adhesion was investigated in a flow chamber fabricated for this purpose.

Figure from

Valentin, J. D., Qin, X.-H., Fessele, C., Straub, H., van der Mei, H. C., Buhmann, M. T., . . . Ren, Q. (2019). Substrate viscosity plays an important role in bacterial adhesion under fluid flow. Journal of colloid and interface science, 552, 247-257.

Biofilm incubator

The contamination-free cultivation of biofilms is a major challenge, especially for microalgal biofilms. In this project, an incubator was developed that allows automated quantification of biofilm growth and microscopy of the biofilms without contaminating them.

Figure from

Buhmann, M., Kroth, P. G., & Schleheck, D. (2012). Photoautotrophic–heterotrophic biofilm communities: a laboratory incubator designed for growing axenic diatoms and bacteria in defined mixed‐species biofilms. Environmental Microbiology Reports, 4(1), 133-140.

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